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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, ICC, IHC, IH(P), WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5682 |
| Replaces | AB9620 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Neurogenin-2 Antibody |
| Alternate Names |
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| Background Information | Neurogenin 2 (NGN2) is a helix-loop-helix class of transcription factor. Transcription factors with bHLH motifs modulate critical events in the development of the mammalian neocortex. The transition from proliferation to neurogenesis involves a coordinate increase in the activity of proneural bHLH factors, including Neurogenin 2 and a decrease in the activity of Hes and ID factors. bHLH factors have key roles in corticogenesis, affecting the timing of differentiation and the specification of cell fate. Neurog2 controls neurogenesis in the embryonic cerebral cortex directly induces the expression of the small GTP-binding protein Rnd2 in newly generated mouse cortical neurons before they initiate migration. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Neurogenin-2 Antibody is validated for use in ELISA, IC, IH, IH(P), WB for the detection of Neurogenin-2. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL using ECL. Immunocytochemistry: Cat. # AB5682 detected Neurogenin-2 in PC12 cells at 1:500 dilution. It was also detected in paraformaldehyde-fixed differentiated human neural stem cells. ELISA: 0.5-1 μg/mL using 1 mg/mL control peptide (Catalog Number AG352) per well. Immunohistochemistry on paraffin sections has been reported by Lamolet, B. et Al. (2004) Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide, amino acids 85-97 of mouse Neurogenin 2. |
| Host | Rabbit |
| Specificity | Recognizes Neurogenin 2 (MATH4a), a Helix-loop-helix class of transcription factor. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Neurogenin-2 is a member of the neurogenin subfamily of basic helix-loop-helix (bHLH) transcription factor genes that play an important role in neurogenesis from migratory neural crest cells.[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q9H2A3 # Involved in neurogenesis (By similarity). SIZE: 272 amino acids; 28621 Da SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein (By similarity). SUBCELLULAR LOCATION: Nucleus (Potential). SIMILARITY: SwissProt: Q9H2A3 ## Contains 1 basic helix-loop-helix (bHLH) domain. |
| Molecular Weight | ~28 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -70ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |