Cayman Thromboxane A Synthase (human, recombinant)

Thromboxane A synthase (TXAS), also known as cytochrome P450 (CYP) isoform CYP5A1, is the enzyme that catalyzes the isomerization of prostaglandin H₂ (PGH₂; Item No. 17020) into thromboxane A₂ (TXA₂), a vasoconstrictor and an inducer of platelet aggregation.^1,2 It also catalyzes the cleavage of PGH₂ into malondialdehyde (MDA) and 12(S)-hydroxyheptadecatrienoic acid (12(S)-HHT; Item No. 34590), a leukotriene B₄ (LTB₄) receptor 2 (BLT₂) agonist that also has a role in platelet aggregation.¹ TXAS exists as a monomer and is composed of an N-terminal membrane anchor domain, a heme-binding catalytic residue, and several substrate-binding residues.^3,4,5 It is expressed in numerous cells, including platelets, monocytes, and macrophages, as well as several tissues, and is localized to the endoplasmic reticulum.³ TXAS undergoes suicide inactivation during catalysis. Mice deficient in TXAS exhibit prolonged bleeding time and defective platelet aggregation.⁶ Caymans Thromboxane A Synthase (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications. To construct this protein, the N-terminal amino acids 1-29 of human CYP5A1 were removed and replaced with a hydrophilic sequence. The F-g loop of TXAS was modified using sequences from rabbit CYP2C5 and CYP2C3. The C-terminal of this protein contains 6x-His tag followed by a stop codon.

WARNING This product is not for human or veterinary use.