Revvity AlphaLISA SureFire Biotin-Free Human Phospho-AKT1/2/3 (Ser473) Detection Kit, 50,000 Assay Points

AKT, also known as Protein Kinase B (PKB), is a serine-threonine kinase that regulates critical cellular processes, including survival and apoptosis. There are three isoforms-AKT1, AKT2, and AKT3-encoded by distinct genes (PKBα, PKBβ, and PKBγ). AKT is activated through phosphorylation at threonine 308 by PDK1 and at serine 473 by mTORC2, following binding of its PH domain to phosphoinositides. Dysregulation of AKT isoforms is linked to various diseases: AKT1 is often amplified in breast and gastric cancers, AKT2 in pancreatic cancer and type 2 diabetes due to its role in glucose uptake, and AKT3 in melanoma and aggressive breast tumors.

The AlphaLISA SureFire Ultra Biotin-Free Human Phospho-AKT1/2/3 (Ser473) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-AKT1/2/3 in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesHigh-throughput screening for preclinical studies - REVPFZ (Additional S&H or Hazmat Fees May Apply)