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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IP, WB, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-257 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-p300 CT Antibody, clone RW128 |
| Alternate Names |
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| Background Information | p300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes. p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function. p300 and CBP are thought to increase gene expression in three ways: (1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity (2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter (3) by acting as adaptor molecules. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in PBS, pH 7.4, 0.05% sodium azide and 30% glycerol |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-fusion to the C-Terminus of human p300 |
| Epitope | C-terminus |
| Clone | RW128 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes p300 and co-immunoprecipitates E1A. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CBP (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CBP can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. |
| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q09472 # Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tats transactivating activity and may help inducing chromatin remodeling of proviral genes. SIZE: 2414 amino acids; 264144 Da SUBUNIT: Interacts with phosphorylated CREB1 (By similarity). Interacts with DTX1, EID1, ELF3, FEN1, LEF1, NCOA1, NCOA6, NR3C1, PCAF, PELP1, SPIB, SRY, TCF7L2, TP53, SRCAP and TRERF1. The TAZ- type 1 domain interacts with HIF1A. Probably part of a complex with HIF1A and CREBBP. Part of a complex containing CARM1 and NCOA2/GRIP1. Interacts with ING4 and this interaction may be indirect. Interacts with the C-terminal region of CITED4. Interacts with HTLV-1 Tax and p30II. Interacts with and acetylates HIV-1 Tat. SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated. & Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1. & Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1. DISEASE: SwissProt: Q09472 # Defects in EP300 may play a role in epithelial cancer. & Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3. & Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. SIMILARITY: SwissProt: Q09472 ## Contains 1 bromo domain. & Contains 1 KIX domain. & Contains 2 TAZ-type zinc fingers. & Contains 1 ZZ-type zinc finger. |
| Molecular Weight | 300 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on HeLa nuclear extract |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |