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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Gt, H, M, R, Sh | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-758 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-phospho-Flt-1 (Tyr1213) Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Immunoaffinity purified rabbit IgG in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-phospho-Flt-1 (Tyr1213) Antibody detects level of phospho-Flt-1 (Tyr1213) & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide containing the sequence (...VR[pY]VN...) in which [pY] corresponds to phospho-tyrosine at residue 1213 of human Flt-1. The immunizing sequence is identical in marmoset, has 14/15 identical amino acids in rat, goat, sheep, cow, and rhesus monkey and 13/15 identical amino acids in mouse. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes phospho-Flt-1 (Tyr1213). |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Oncogene FLT belongs to the src gene family and is related to oncogene ROS (MIM 165020). Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The sequence structure of the FLT gene resembles that of the FMS gene (MIM 164770); hence, Yoshida et Al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.[supplied by OMIM] |
| Gene Symbol |
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| Modifications |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P17948 # Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. SIZE: 1338 amino acids; 150769 Da SUBUNIT: Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1. SUBCELLULAR LOCATION: Isoform Flt1: Cell membrane; Single-pass type I membrane protein. & Isoform sFlt1: Secreted. TISSUE SPECIFICITY: Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta. SIMILARITY: SwissProt: P17948 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. |
| Molecular Weight | ~180 kDa (66 kDa for the fragment tested in QC) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on recombinant Flt-1 (Catalog # 14-562) phosphorylated upon incubation with ATP |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |