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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1248 |
| Replaces | MAB3526 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cytochrome P450 Enzyme CYP1A2 Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit antiserum. Liquid. No preservatives |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cytochrome P450 Enzyme CYP1A2 Antibody is an antibody against Cytochrome P450 Enzyme CYP1A2 for use in IH & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000-1:4,000 Immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide |
| Host | Rabbit |
| Specificity | Reacts with human cytochrome P450 enzyme CYP1A2 in hepatic microsomal fraction. No cross-reactivity with other cytochrome P450 enzymes. By Western blot the antibody recognizes a protein with a molecular weight of approximately 54 kDa. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzymes endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3 untranslated region. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05177 # Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. SIZE: 515 amino acids; 58294 Da SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. TISSUE SPECIFICITY: Liver. SIMILARITY: SwissProt: P05177 ## Belongs to the cytochrome P450 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |