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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IP, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 06-966 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-CDK1/CDC2 Antibody (C-Term) |
| Alternate Names |
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| Background Information | Entry of all eukaryotic cells into mitosis is regulated by activation of CDC2 kinase. Activation of CDC2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating CDC2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of CDC2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of CDC2 is carried out by the CDC25 phosphatase. Expression of CDC2 plays a critical role in cell transition through the G2/M phase. The activity of CDC2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for CDC2. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | rabbit antiserum containing 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Cdk1/Cdc2 with Anti-Cdk1/Cdc2 Antibody, CT (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 5 µL of this antibody immunoprecipited CDK1/CDC2 (C-terminus) from Balb/3T3/A31 cell lysate. Immunoprecipited sample was then subject to Western Blotting analysis. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a region near the near the C-terminus of human CDK1/CDC2. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Predicted to react with Hamster, Rat, and Human with greater than 90% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Antiserum |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. SIZE: 297 amino acids; 34095 Da SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. SUBCELLULAR LOCATION: Nucleus (By similarity). SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain. |
| Molecular Weight | 34kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Balb/3T3/A31 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDK1/CDC2 (C-terminus) in Balb/3T3/A31 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |