| Replacement Information | |
|---|---|
| Replacement Information | 07-131 is a recommended replacement for 05-707 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, ICC, ChIP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-131 |
| Replaces | 05-707 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-Sirt1(Sir2) Antibody |
| Alternate Names |
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| Background Information | NAD-dependent protein deacetylase sirtuin-1 (UniProt: Q923E4; also known as Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, Sirt-1) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID: 93759) in murine species. Sirt1 is a NAD-dependent histone deacetylase that is widely expressed and links transcriptional regulation directly to intracellular energetics. In liver, Sirt1 is expressed in a circadian manner with maximal and minimal levels reaching at around Zeitgeber time (ZT) 16 and ZT4, respectively and its deacetylase activity is also regulated in a circadian manner that peaks at ZT15. It participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy. It is shown to modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Sirt1 deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression in both positive and negative manner. It serves as a sensor of the cytosolic ratio of NAD+/NADH, which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Sirt1 has four nucleotide (NAD)-binding regions and four zinc binding sites. It is reported to shuttle between nucleus and cytoplasm. Sirt1 is proteolytically cleaved by cathepsin B upon TNF treatment to yield a catalytic inactive but stable 75 kDa fragment (aa 2-525). Sirt1 can be phosphorylated by multiple protein kinases. It is phosphorylated by STK4/MST1 that results in inhibition of Sirt1-mediated p53/TP53 deacetylation and phosphorylation by MAPK8/JNK1 at serine 46 and threonine 522 is reported to increase its nuclear localization and enzymatic activity. Sirt1 phosphorylation at threonine 522 by DYRK1A and DYRK3 activates its deacetylase activity and can promote cell survival. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
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| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide with 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Sirt1(Sir2), Cat. No. 07-131, is a rabbit polyclonal antibody that detects Sirt1/Sir2 and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, and Western Blotting. |
| Key Applications |
|
| Application Notes | Tested Applications Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Sirt1 in HeLa, A431, HepG2, and NIH3T3 cells. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant fragment corresponding to the first 131 amino acids from Mouse Sirt1 protein. |
| Epitope | a.a. 1-131 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects NAD-dependent protein Sirt1/Sir2. It targets an epitope within the N-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene. |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Essential during embryogenesis and gametogenesis. Deacetylates Lys-382 of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit (By similarity). ENZYME REGULATION: Activated by resveratrol (3,5,4-trihydroxy-trans-stilbene), butein (3,4,2,4-tetrahydroxychalcone), piceatannol (3,5,3,4-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2,4-trihydroxychalcone), fisetin (3,7,3,4-tetrahydroxyflavone) and quercetin (3,5,7,3,4-pentahydroxyflavone) (By similarity). Inhibited by nicotinamide. SUBUNIT: Interacts with MLLT7/FOXO4, HES1, HEY2 and PML (By similarity). Found in a complex with PCAF and MYOD1. Interacts with p53/TP53. Interacts with TAF1B. SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Recruited to the nuclear bodies via its interaction with PML (By similarity). TISSUE SPECIFICITY: Widely expressed. Weakly expressed in liver and skeletal muscle. SIMILARITY: Belongs to the sirtuin family. SIMILARITY: Contains 1 deacetylase sirtuin-type domain. |
| Molecular Weight | Target molecular weight ~100 kDa observed; 80.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in nuclear extract of BALB/3T3 clone A31. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Sirt1 in nuclear extract of BALB/3T3 clone A31. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 μL |