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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Gp, B, M, PO, Av | FC, ICC, IF, WB | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 16-232 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 488 conjugate |
| Alternate Names |
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| Background Information | Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Alexa Fluor® 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide. Liquid at 2-8°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells. Immunocytochemistry: 2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE). |
| Epitope | The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE). |
| Clone | DM1A |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION:SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE:451 amino acids; 50136 Da SUBUNIT: Dimer of alpha and beta chains. PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity). SIMILARITY:SwissProt: Q71U36 ## Belongs to the tubulin family. |
| Molecular Weight | 50-60 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Flow Cytometry. Flow Cytometry: 0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Do Not Freeze. Do not store the material diluted. For maximum recovery of product, centrifuge original vial prior to removing cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |