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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| PO, R | ELISA, IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1765P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Dopamine D1A Receptor Antibody, CT, cytoplasmic |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Dopamine D1A Receptor Antibody, C-terminus, cytoplasmic is an antibody against Dopamine D1A Receptor for use in ELISA, IH & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-10 μg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang et Al. 1992; OConnel et Al. 1995). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band (Huang et Al. 1992; OConnel et Al. 1995) that is present in relatively small amounts. Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections. ELISA: 0.5-1.0 μg/mL (1 μg/mL D1A peptide (Cat. Number AG259)/well) Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | A 13 amino acid peptide sequence (amino acids 403-415) from rat D1A (Monsma et Al. 1990; Zhou et Al. 1990; Zhou et Al. 1992; ODowd et Al. 1990). This peptide is predicted to be cytoplasmic. |
| Epitope | C-terminus, cytoplasmic |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Dopamine Receptor D1A. An antibody made to the D1R peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes by Western blot. The 75 kDa band was not visible in striatum (Huang et Al. 1992). Approximately 100-400 μg of protein/lane should be loaded to visualize the 75 kDa band. By immunohistochemistry D1R has been localized in various regions of the rat brain (Huang et Al. 1992). May recognize porcine or bovine (100 % similarity) or human (78% similarity). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This g-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P21728 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by g proteins which activate adenylyl cyclase. SIZE: 446 amino acids; 49293 Da SUBUNIT: Interacts with DNAJC14 via its C-terminus (By similarity). Interacts with DRD1IP. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14 (By similarity). TISSUE SPECIFICITY: Detected in caudate, nucleus accumbens and in the olfactory tubercle. SIMILARITY: SwissProt: P21728 ## Belongs to the g-protein coupled receptor 1 family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |