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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H, Mk | ELISA, IF, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5062P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Vesicular GABA Transporter (VGAT) Antibody |
| Alternate Names |
|
| Background Information | Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH for the detection of Vesicular GABA Transporter (VGAT). |
| Key Applications |
|
| Application Notes | Immunohistochemistry/Immunofluorescence: 2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections. ELISA: 1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997). |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins. |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9H598 # Involved in the uptake of GABA and glycine into the synaptic vesicles. SIZE: 525 amino acids; 57415 Da SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (By similarity). TISSUE SPECIFICITY : Retina. Expressed throughout the horizontal cells or more specifically at the terminals. SIMILARITY: SwissProt: Q9H598 ## Belongs to the amino acid/polyamine transporter 2 family. |
| Molecular Weight | 57 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |