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Millipore Anti-Phospho-Dbc1 (Thr454) Antibody Is An Antibody Against Phospho-Dbc1 (Thr454) For Use In Western Blotting, Peptide Inhibition Assay. - Mill (Additional S&H or Hazmat Fees May Apply)
Deleted in breast cancer gene 1 protein (DBC1) is a large, nuclear protein with multiple functions in cell survival that binds directly to the estrogen receptor α (ERα) hormone-binding domain in a strict ligand-independent manner. DBC1 promotes p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity. It is ubiquitously expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
Product Information
Format
Affinity Purified
Control
Untreated and etoposide treated Jurkat cell lysates
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-phospho-DBC1 (Thr454) Antibody is an antibody against phospho-DBC1 (Thr454) for use in Western Blotting, Peptide Inhibition Assay.
Key Applications
Western Blotting
Peptide Inhibition Assay
Application Notes
Peptide Inhibition Assay: A 1:500 dilution from a representative lot blocked DBC-1 phosphorylated at Thr454 in untreated and etoposide treated Jurkat cell lysates.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human DBC-1 phosphorylated at Thr454.
Epitope
Phosphorylated Thr454
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This antibody recognizes DBC1 phosphorylated at Thr454.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_066997
Gene Symbol
KIAA1967
DBC1
Purification Method
Affinity Purfied
UniProt Number
Q8N163
Molecular Weight
~130 kDa observed. Uniprot states the calculated molecular weight is 103 kDa but has been shown at ~130kDa (Satoh, M., et Al. (1995). Brain Res Dev Brain Res. 87(2):111-119).
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in untreated and etoposide treated Jurkat cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected DBC-1 phosphorylated at Thr454 in 10 µg of etoposide treated Jurkat cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
