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Millipore Goat Anti-Rabbit Igg Antibody, (H+L) Fitc Conjugate Detects Level Of Rabbit Igg & Has Been Published & Validated For Use In If. - Mill (Additional S&H or Hazmat Fees May Apply)
The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to fluorescein isothiocyanate (FITC) and stabilized in buffer
Background Information
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
Format
FITC
Presentation
Immunoaffinity Purified immunoglobulin in 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA containing no preservatives.
Quality Level
MQ100
Applications
Application
Goat anti-Rabbit IgG Antibody, (H+L) FITC conjugate detects level of Rabbit IgG & has been published & validated for use in IF.
Key Applications
Immunofluorescence
Application Notes
Immunohistochemistry:1:200-1:500 (1-4)
Immunocytochemistry: 1:200-1:500 (1,4)
Flow Cytometry: 1 μg per 1x10E6 cells (1,2,4)
Optimal working dilutions must be determined by the end user.
Biological Information
Concentration
1.0 mg/mL
Host
Goat
Specificity
Specific for rabbit IgG, heavy and light chain. The cross-reactivities of anti-rabbit IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
Species Reactivity
Rabbit
Antibody Type
Polyclonal Antibody
Purification Method
ImmunoAffinity Purified
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
The undiluted antibody is stable at 2–8°C for 12 months. Do not store in a diluted format.