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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, Rb | WB, IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL218 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Keratin K3/K76 Antibody, clone AE5 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry on frozen sections: 1:50. Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004). Western blotting: 1:500. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Rabbit corneal epithelial keratin |
| Clone | AE5 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmanns Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
| Gene Symbol |
|
| Purification Method | Ion-exchange chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q01546 # Probably contributes to terminal cornification. SIZE: 638 amino acids; 65871 Da SUBUNIT: Heterotetramer of two type I and two type II keratins. DEVELOPMENTAL STAGE: Synthesized during maturation of epidermal keratinocytes. SIMILARITY: SwissProt: Q01546 ## Belongs to the intermediate filament family. MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). |
| Molecular Weight | 64 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |