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Millipore Detect Cd43 Using This Milli-Mark Anti-Cd43-Fitc Antibody, Clone Df-T1 Validated For Use In Fc. - Mill (Additional S&H or Hazmat Fees May Apply)
CD43 is a human heavily 0-glycosylated transmembrane protein, which is expressed by T cells and myeloid lineage cells (Borche, 1987; Stross, 1989). Reactivity with plasma cells has been observed (Beschomer, 1999). The molecule is known to be defective in the Wiskott-Aldrich syndrome and may participate in T-cell activation (Stross, 1989). Immunoblotting studies have revealed molecular mass heterogeneity displayed by the antigen extracted from white blood cells of different lineage. Thus, two major bands of 140 and 130 kDa are seen for the promyelocytic cell line HL60, 110 kDa for the myeloblast cell line KG1 and a major 115 kDa band for peripheral blood lymphocytes (Stross, 1989).
Product Information
Format
FITC
Control
PBMCs
Presentation
Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Quality Level
MQ100
Applications
Application
Detect CD43 using this Milli-Mark Anti-CD43-FITC Antibody, clone DF-T1 validated for use in FC.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Myeloblast cell line KG1
Clone
DF-T1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Antibody recognizes Human CD43 antigen.
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001025459.1
Purification Method
Protein A Purfied
UniProt Number
P16150
Molecular Weight
110 kDa (Stross, 1989)
Product Usage Statements
Quality Assurance
Evaluated by flow cytometry using human PBMCs
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
