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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB2014 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-OB-Cadherin Antibody, extracellular domain, clone 16A |
| Alternate Names |
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| Background Information | Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Affinity purified immuoglobulin. Liquid in PBS containing 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect OB-Cadherin using this Anti-OB-Cadherin Antibody, extracellular domain, clone 16A validated for use in WB, IC, IH. |
| Key Applications |
|
| Application Notes | Western blot: 1:25 - 1:250 Immunohistochemistry: Frozen tissue sections. 1:25 - 1:50 dilution using avidin-biotinylated horseradish peroxidase complex (ABC) as detection protocol. Immunocytochemistry: Methanol and paraformaldehyde-fixed cells |
| Biological Information | |
|---|---|
| Immunogen | Fusion protein corresponding to extracellular domain of human OB-cadherin coupled to GST. |
| Epitope | extracellular domain |
| Clone | 16A |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the extracellular domain of human cadherin-11. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P55287 # Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. SIZE: 796 amino acids; 87965 Da SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts. SIMILARITY: SwissProt: P55287 ## Contains 5 cadherin domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at 4°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |