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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | ELISA, WB, IH(P) | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB463 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Estrogen Receptor Antibody, clone B10 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | Ascites. Liquid, does not contain any preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000 Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000 ELISA at 1:1,000-1:2,000 Western blot analysis at 1:1,000-1:2,000 Immunoprecipitation at 1:1-1:10 Gel super shifts at 1:1-1:10 Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Epitope | a.a. 151-165 |
| Clone | B10 |
| Host | Mouse |
| Specificity | The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors. SPECIES REACTIVITIES: Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |