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Millipore Detect Mucin Using This Mouse Monoclonal Antibody, Anti-Muc13 Antibody, Clone 2e11.1 Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Mucin-13 (MUC-13) is also called Down-regulated in colon cancer 1 (DRCC1). MUC-13 is an epithelial and hemopoietic transmembrane mucin that is likely involved in cell signaling. MUC-13 is highly expressed in epithelial tissues (especially in gastrointestinal and respiratory tracts) as well as kidney, small intestine, appendix and stomach.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Mucin using this mouse monoclonal antibody, Anti-MUC13 Antibody, clone 2E11.1 validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected MUC-13 in 10 µg of human kidney tissue lysate.
Immunohistochemistry Analysis: a 1:500 dilution from a representative lot detected MUC-13 in human ilium tissues.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human MUC-13.
Epitope
This antibody recognizes the cytoplasmic domain within the Beta subunit of MUC-13.
Clone
2E11.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_149038
Gene Symbol
MUC13
DRCC1
RECC
Purification Method
Protein g Purified
UniProt Number
Q9H3R2
Molecular Weight
~33 and ~60 kDa observed. Swiss-Prot describes a molecular weight of ~55 kDa. Due post-translational modification, this protein is cleaved resulting in beta subunit (~33 kDa) and an alpha subunit (Williams, S. J., et Al. (2001). J Biol Chem. 276(21):18327-18336. Maher, D. M., et Al. (2011). Mol Cancer Res. 9(5):531-537.). Uncharacterized band(s) may be observed in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human small intestine tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MUC-13 in 10 µg of human small intestine tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
