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Millipore This Rat Monoclonal Anti-F4/80 Antibody (Mouse), Apc, Clone Bm8.1, Cat. No. Mabf1523 Is Validated For Use In Flow Cytometry, For The Detection Of F4/80. - Mill (Additional S&H or Hazmat Fees May Apply)
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.
Product Information
Format
APC
Presentation
Purified Rat monoclonal containing 10 mm NaH2PO4, 150 mm NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality Level
MQ100
Applications
Application
This rat monoclonal Anti-F4/80 Antibody (mouse), APC, clone BM8.1, Cat. No. MABF1523 is validated for use in Flow Cytometry, for the detection of F4/80.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
BM8.1
Concentration
Please refer to lot specific datasheet.
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Emr1
Gpf480
Purification Method
Purified by size-exclusion chromatography
UniProt Number
Q61549
Product Usage Statements
Quality Assurance
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 μg from a representative lot detected F4/80 in one million C57Bl/6 splenocytes stained with FITC Anti-mouse CD11b.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
