Cell Signaling Phospho-Fadd (Ser194) Antibody (Human Specific)

Name: Cell Signaling Phospho-Fadd (Ser194) Antibody (Human Specific)
SKU: CSIG-2781S
Price: 365.62 USD

List Price $365.62 Your Price $365.62

Cell Signaling Phospho-Fadd (Ser194) Antibody (Human Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2781S

MFG.PART: 2781S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	28
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-FADD (Ser194) Antibody detects endogenous levels of human FADD protein only when phosphorylated at serine 194.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser194 of human FADD. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8 (1,2). FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent (3) and may be related to its regulatory role in embryonic development and cell cycle progression (4,5).

Specifications