Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABE570-25UG |
| Description | Anti-RINF (CXXC5) |
| Alternate Names |
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| Background Information | CXXC-type zinc finger protein 5 (UniProt: Q7LFL8; also known as CF5, Putative MAPK-activating protein PM08, Putative NF-kappa-B-activating protein 102, Retinoid-inducible nuclear factor, RINF) is encoded by the CXXCS (also known as HSPC195, TCCCIA00297) gene (Gene ID: 51523) in human. In human cells, two isoforms of RINF have been reported that are produced by alternative splicing. The isoform two lacks the first 95 amino acids. RINF serves as a transcription factor that binds to the oxygen responsive element of COX4I2 and represses its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen). Its nuclear localization sequence is localized in amino acids 257-262. It is shown to co-localize with DVL1 in large bodies localized outside the nuclear membrane. RINF is shown to be important in normal myelopoiesis. RINF acts as a mediator of BMP4-mediated modulation of canonical Wnt signaling activity in neural stem cells. It is also shown to be essential for DNA damage-induced ATM phosphorylation, p53 activation, and cell cycle arrest. RINF silencing is reported to increase sensitivity of K562 cells to daunorubicin- and lenalidomide. (Ref.: Aston A et Al. (2013). Oncotarget. 4(9); 1438-1448). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-RINF (CXXC5) Antibody, Cat. No. ABE570, is a highly specific rabbit polyclonal antibody that targets RINF (CXXC5) and has been tested in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human CXXC-type zinc finger protein 5. Immunogen is conserved between two isoforms. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects CXXC-type zinc finger protein 5 (RINF) in human cells. It targets an epitope within 17 amino acids from the N-terminal half. |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Mouse and Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~35 kDa observed; 32.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected RINF (CXXC5) in 10 µg of MCF-7 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |