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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF235 |
| Description | Anti-AIRE Antibody, clone Mab155 |
| Alternate Names |
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| Background Information | AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-AIRE Antibody, clone Mab155 is validated for use in Flow Cytometry and Western Blotting and Immunocytochemistry for the detection of AIRE. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected AIRE in Cos1 transfected cell lysate (Rinderle, C., et Al. (1999). Human Molecular Genetics. 8(2):277-290). Immunocytochemistry Analysis: A representative lot detected AIRE in freshly isolated endogenous peripheral blood lymphocytes (Rinderle, C., et Al. (1999). Human Molecular Genetics. 8(2):277-290). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein corresponding to human AIRE. |
| Clone | Mab155 |
| Concentration | 1 mg/mL |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | 58 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in human PBMC cells. Flow Cytometry Analysis: 0.5 µg of this antibody detected AIRE in human PBMC cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |