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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, FC, ICC, IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF244 |
| Description | Anti-PAR-1, clone ATAP2 Antibody |
| Alternate Names |
|
| Background Information | The protein named Proteinase-activated receptor 1 (PAR-1/PAR1) or Coagulation factor II receptor, or Thrombin receptor and encoded by the human gene F2R/CR2R/PAR1/TR is a high affinity receptor for activated thrombin and part of the innate immune system response driven by proteinase-activated receptors (PARs). PARs are g protein-coupled receptors that transmit cellular responses to extracellular proteases and have important functions in vascular physiology, development, inflammation, and cancer progression. PAR1 is important for platelet activation and vascular development. PAR1 expression is limited to platelets and vascular endothelial cells and is localized to the cell membrane. Interestingly too, even the signal peptide of PAR1 (which guides the protein to the membrane) plays a role in cell signaling as if forms the intracellular angiogenesis inhibitor peptide Parstatin. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PAR-1 antibody, clone ATAP2 is validated for use in western blotting, flow cytometry, ICC & immunofluorescence for the detection of PAR-1. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 4 µg from a representative lot detected Par-1 in 1X10E6 human thrombocytes (platelets). Flow Cytometry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et Al. (1993). J Biol Chem. 268(18):13756-13763.). Western Blotting Analysis: A representative lot from an independent laboratory detected Par-1 in human platelet membrane cell lysate (Brass, L. F., et Al. (1992). J Biol Chem. 267(20):13795-13798.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et Al. (1993). J Biol Chem. 268(18):13756-13763.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 cells (Brass, L. F., et Al. (1994). J Biol Chem. 269(4):2943-2952.). |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human Par-1. |
| Clone | ATAP2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse lymph node tissue. Western Blotting Analysis: 1 µg/mL of this antibody detected Par-1 in 10 µg of mouse lymph node tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |