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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, IHC, FC | M | APC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF557 |
| Description | Anti-CD28 Antibody (human), APC, clone CD28.2 |
| Alternate Names |
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| Background Information | The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo. This antibody is also reported to be cross-reactive with several non-human species, including Baboon, Chimpanzee, Cynomolgus, and Rhesus. |
| Product Information | |
|---|---|
| Format | APC |
| Presentation | Purified mouse monoclonal antibody in buffer containing 10 mm NaH2PO4, 150 mm NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This mouse monoclonal antibody Anti-CD28 (human), APC & clone CD28.2 validated for use in IP;IHC;Flow Cytometry for the detection of CD28. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Immunogen information for this antibody is proprietary information. |
| Clone | CD28.2 |
| Concentration | 5 µL (0.25 µg)/test |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Size Exclusion Chromatography |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 0.25 µg of this antibody detected CD28 in one million human peripheral blood lymphocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Packaging Information | |
|---|---|
| Material Size | 25 tests |