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Millipore This Anti-Lims1, Clone 5g7 Antibody Is Validated For Use In Western Blotting, ELISA, Flow Cytometry & Immunofluorescence For The Detection Of Lims1. - Mill (Additional S&H or Hazmat Fees May Apply)
LIM and senescent cell antigen-like-containing domain protein 1
Particularly interesting new Cys-His protein 1
PINCH-1
Renal carcinoma antigen NY-REN-48
Background Information
LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1), also known as Particularly interesting new Cys-His protein 1 (PINCH-1; PINCH1; PINCH), or Renal carcinoma antigen NY-REN-48 is an adaptor protein which contains five LIM domains, or double zinc fingers. LIMS1 is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that Lims1 may play a role in integrin-mediated cell adhesion or spreading.
Product Information
Format
Ascites
Presentation
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-LIMS1, clone 5G7 Antibody is validated for use in western blotting, ELISA, flow cytometry & immunofluorescence for the detection of LIMS1.
Key Applications
Western Blotting
ELISA
Flow Cytometry
Immunofluorescence
Application Notes
ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected LIMS1 in HeLa cells.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected LIMS1 in HepG2 cells.
Biological Information
Immunogen
Purified recombinant fragment of human LIMS1 expressed in E. Coli
Clone
5G7
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
LIMS1
PINCH
PINCH1
Purification Method
Unpurified
UniProt Number
P48059
Molecular Weight
~37 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A549, Jurkat and HeLa cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected LIMS1 in A549, Jurkat and HeLa cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
