Cell Signaling Hsp27 (E1j4d) Rabbit mAb (Ihc Preferred)

Name: Cell Signaling Hsp27 (E1j4d) Rabbit mAb (Ihc Preferred)
SKU: CSIG-50353S
Price: 354.51 USD

List Price $354.51 Your Price $354.51

Cell Signaling Hsp27 (E1j4d) Rabbit mAb (Ihc Preferred) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-50353S

MFG.PART: 50353S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	27
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:800}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	HSP27 (E1J4D) Rabbit mAb recognizes endogenous levels of total HSP27 protein.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HSP27 protein.
Background	Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

Specifications