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Millipore In Vivo Shape Reagent For Live Cell Rna Structure Analysis
Millipore In Vivo Shape Reagent For Live Cell Rna Structure Analysis
List Price$303.97Your Price$303.97
Millipore In Vivo Shape Reagent Contains 2-Methylnicotinic Acid Imidazolide (Nai). This Reagent Has Very Low Toxicity And Permits The Analysis Of Rna Structure In Living Cells Using - Mill (Additional S&H or Hazmat Fees May Apply)
In Vivo SHAPE Reagent for Live Cell RNA Structure Analysis
Overview
Features & Advantages
Ready-to-use RNase-free solution
Low toxicity allows use with live cells
High cell-permeability for rapid modification of RNA
Reacts with all RNA nucleotides without bias for high resolution mapping
In Vivo SHAPE Reagent is used for the determination of RNA structure. This reagent contains a highly pure form of 2-methylnicotinic acid imidazolide (NAI). This compound forms 2’-O-adducts on single stranded regions of RNA that can be detected by Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).
In contrast to other reagents used for the SHAPE method, the NAI–based In Vivo SHAPE Reagent is rapidly absorbed into cells and is formulated to have low levels of toxicity making it ideal for use with live cells. Unlike other reagents used for SHAPE analysis, In Vivo SHAPE Reagent reacts in an unbiased fashion with all four RNA nucleotides. This combination of low toxicity, rapid uptake and lack of nucleotide bias enables high resolution RNA structure analysis in a variety of living systems.
Background Information
Folding into higher-order structures allows RNAs to function inside living cells and form dynamic complexes with effector proteins. Predicting these 3D structures is challenging and therefore a number of probing techniques have been developed to study RNA folding.
Compounds such as 2-methylnicotinic acid imidazolide (NAI) contained the In Vivo SHAPE Reagent result in the formation of 2’-O-adducts. These adducts form rapidly on single stranded unpaired RNA and much more slowly on based paired (double stranded) RNA. Modified RNA can then be analyzed by primer extension. Modified nucleotides are not extended by RNA dependent DNA polymerases thus allowing sites of modification to be detected.
References 1. Wilkinson K., et al. (2006). Nature Protocols,1(3): 1610-1616 2. Spitale R., et al. (2012). Nature Chemical Biology. 9(1):18-20
Product Information
Components
2-methylnicotinic acid imidazolide (NAI) in DMSO
Formulation
Presentation
0.5 mL vial containing 2M NAI (>97% pure) in DMSO solution
Quality Level
MQ100
Applications
Application
In Vivo SHAPE Reagent contains 2-methylnicotinic acid imidazolide (NAI). This reagent has very low toxicity and permits the analysis of RNA structure in living cells using Selective 2’-Hydroxyl Acylation and Primer Extension (SHAPE).
Key Applications
Reverse transcriptase assays
Biological Information
Concentration
2M
Product Usage Statements
Quality Assurance
Purity of NAI compound is tested by HPLC.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store NAI at -20°C in a non-frost-free freezer protected from light. With proper storage (protect from light and avoid excessive freeze-thaw cycles) this product is stable for up to 6 months from the date of receipt.
