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Millipore Active, N-Terminal His6-Tagged Recombinant Human Gsk3β With An H350l Mutation, For Use In Kinase Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
N-terminal His6-tagged recombinant human GSK3β with an H350L mutation
Product Information
Quality Level
MQ100
Applications
Application
Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.
Key Applications
Kinase Assay
Biological Information
Source
Expressed by baculovirus in Sf21 insect cells
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_002093.2
Entrez Gene Summary
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]
Gene Symbol
GSK3B
Protein Target
GSK3β
Purification Method
Ni2+/NTA-agarose
Target Sub-Family
CMGC
UniProt Number
P49841
UniProt Summary
FUNCTION: SwissProt: P49841 # Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. SIZE: 420 amino acids; 46744 Da SUBUNIT: Monomer (By similarity). Interacts with NIN and CABYR. TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. PTM: Phosphorylated by AKT1 and ILK1. SIMILARITY: SwissProt: P49841 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
Molecular Weight
51kDa
Product Usage Statements
Quality Assurance
Routinely evaluated by phosphorylation of Phospho-Glycogen Synthase Peptide-2
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
6 months at -70°C
Packaging Information
Material Size
10 µg
Material Package
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.
