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Millipore Active, N-Terminal, His6-Tagged, Recombinant Human Pdk1, Amino Acids 52-End. For Use In Kinase Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.
Key Applications
Kinase Assay
Biological Information
Source
Human PDK1 expressed by baculovirus in Sf21 insect cells
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_002613.3
NM_031268.4
Gene Symbol
PDPK1
MGC20087
PDK1
PkB-like
PRO0461
MGC35290
hPDK1
Protein Target
PDK1
Purification Method
Ni2+/NTA-agarose
Target Sub-Family
AGC
UniProt Number
O15530
UniProt Summary
FUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. SIZE: 556 amino acids; 63152 Da SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. TISSUE SPECIFICITY: Appears to be expressed ubiquitously. PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight
59kDa
Product Usage Statements
Quality Assurance
Routinely evaluated by phosphorylation of PDKtide
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
6 months at -70°C
Packaging Information
Material Size
10 µg
Material Package
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.
