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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Rhesus Macaque, Chp, R | WB, IH(P) | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABN66 |
| Description | Anti-Cholesterol acyltransferase 1 Antibody |
| Alternate Names |
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| Background Information | Cholesterol acyltransferase 1 (ACAT1) is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimers disease. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cholesterol acyltransferase 1 in human adrenal gland and normal human brain tissues. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Cholesterol acyltransferase 1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Rhesus Macaque, Chimpanzee, and Rat based on 100% sequence homology. Other homologies: Mouse (93% sequence homology). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Acyl-coenzyme A:cholesterol acyltransferase (ACACT; EC 2.3.1.26) is an intracellular protein located in the endoplasmic reticulum that forms cholesterol esters from cholesterol. Accumulation of cholesterol esters as cytoplasmic lipid droplets within macrophages and smooth muscle cells is a characteristic feature of the early stages of atherosclerotic plaques (Cadigan et al., 1988). |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. CATALYTIC ACTIVITY: Acyl-CoA + cholesterol = CoA + cholesterol ester. SUBUNIT STRUCTURE: May form homo- or heterodimers. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. INDUCTION: Highly activated by the presence of cholesterol. SEQUENCE SIMILARITIES: Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. |
| Molecular Weight | ~58 kDa observed which may be the result of dimerization/aggregation of the native protein during sample preparation for SDS-PAGE. The calculated molecular weight is 65 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Caco-2 cell lysate. Western Blot Analysis: 0.05 µg/mL of this antibody detected Cholesterol acyltransferase 1 on 10 µg of Caco-2 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |