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What is your starting material?
RNA
What do you value most in a reverse transcriptase?
Accuracy
What is your preferred format?
Stand Alone Enzyme
Recommendation:
AccuScript High-Fidelity Reverse Transcriptase
Product Details
- Highest reverse transcription accuracy with 3-6x fewer errors
- Full length cDNA up to 20 kb
- Up to 3x faster RT-PCR reaction times
Ordering Info
| Size | Catalog |
|---|---|
| 50 rxn | 600089 |
| 200 rxn | 600090 |
RT-PCR Kit
Recommendation:
AccuScript PfuUltra II RT-PCR Kit
Product Details
- Complete system for RT-PCR applications that require high-fidelity, specificity & throughput
- Amplifies cDNA targets up 9.6 kb in length
- Synthesize cDNA from 10-1000 ng of total RNA or 0.1-10 ng of poly(A)+ RNA
- Up to 3x faster RT-PCR reaction times
Ordering Info
| Size | Catalog |
|---|---|
| 50 rxn | 600184 |
Robustness/Yield
What is your preferred format?
Stand Alone Enzyme
Recommendation:
AffinityScript Multiple Temperature Reverse Transcriptase
Product Details
-
Robust cDNA synthesis across a broad temperature range,
37-55°C - High affinity for primer-template complexes promotes optimal yield
- Synthesize long cDNA targets, up to 20 kb
- Detects low input RNA
Ordering Info
| Size | Catalog |
|---|---|
| 10 rxn | 600105 |
| 50 rxn | 600107 |
| 200 rxn | 600109 |
RT-PCR Kit
Recommendation:
AffinityScript One-Step RT-PCR Kit
Product Details
- Reduces cycling times & contamination risk with a one-tube, master mix format
- High affinity for primer-template complexes promotes optimal yield
- Robust performance for difficult and GC-rich targets
- System optimized for target lengths up to 9.5 kb
Ordering Info
| Size | Catalog |
|---|---|
| 100 rxn | 600188 |
DNA
Select the enzyme category that best fits your needs:
High-Fidelity (Proofreading)
Select the best description for your target:
GC-rich or long templates
Recommendation:
Herculase II Fusion DNA Polymerases
Product Details
- Superior yield for routine PCR applications & difficult/GC-rich targets
- Fast cycling time with 15 sec/kb extension rate
- Accurately amplifies up to 23 kb genomic DNA targets
- Preserves Pfu fidelity, 1 error/770,000 bp
- High sensitivity for amplification of low abundance targets
Ordering Info
| Size | Catalog |
|---|---|
| 40 rxn | 600675 |
| 200 rxn | 600677 |
| 400 rxn | 600679 |
Template contains Uracil and/or dUTP in the PCR Reaction
Recommendation:
PfuTurbo Cx HotStart DNA Polymerase
Product Details
- World's only high-fidelity polymerase that reads through and incorporates dUTP
- Great for bi-sulfite sequencing, UNG decontamination protocols & DNA methylation studies
- Accurately amplifies up to 10 kb genomic DNA templates, including GC-rich targets
- DMSO provided separately to assist in optimizing PCR conditions
Ordering Info
| Size | Catalog |
|---|---|
| 100 U | 600410 |
| 500 U | 600412 |
| 1000 U | 600414 |
PCR product intended for cloning
Would you prefer the most accurately amplified PCR products or products
ready for TA/UA cloning?
ready for TA/UA cloning?
Most Accurate (Highest Fidelity)
What is your preferred format?
Hot Start Master Mix
Recommendation:
PfuUltra II HotStart Master Mix
Product Details
- Highest fidelity for the most reliable, error-free PCR
- Fast cycling time with 15 sec/kb extension rate
- Accurately amplifies up to 19 kb genomic DNA targets
- Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
| Size | Catalog |
|---|---|
| 100 rxn | 600850 |
| 400 rxn | 600852 |
Hot Start Enzyme
Recommendation:
PfuUltra II Fusion HotStart DNA Polymerase
Product Details
- Highest fidelity for the most reliable, error-free PCR
- Fast cycling time with 15 sec/kb extension rate
- Accurately amplifies up to 19 kb genomic DNA targets
- Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
| Size | Catalog |
|---|---|
| 40 rxn | 600670 |
| 200 rxn | 600672 |
| 400 rxn | 600674 |
Most Convenient (TA/UA Cloning
Ready)
Ready)
What is your preferred format?
Hot Start Master Mix
Recommendation:
Easy-A High-Fidelity PCR Master Mix
Product Details
- Combines accuracy of Pfu with TA/UA cloning efficiency of Taq
- Preferentially adds a single 3’-A overhang to PCR products
- PCR products have greater than 95% cloning efficiency
Ordering Info
| Size | Catalog |
|---|---|
| 100 rxn | 600640 |
| 400 rxn | 600642 |
Hot Start Enzyme
Recommendation:
Easy-A High-Fidelity PCR Cloning Enzyme
Product Details
- Combines accuracy of Pfu with TA/UA cloning efficiency of Taq
- Preferentially adds a single 3’-A overhang to PCR products
- PCR products have greater than 95% cloning efficiency
Ordering Info
| Size | Catalog |
|---|---|
| 100 U | 600400 |
| 500 U | 600402 |
| 1000 U | 600404 |
Minimal template DNA (low copy number, archaic, precious samples)
Recommendation:
PicoMaxx High-Fidelity PCR System
Product Details
- Highest sensitivity to detect low copy number targets
- Robustly withstands the effects of PCR inhibitors
- Robust amplification of genomic DNA targets up to 10 kb
- High-specificity hot start increases enzyme sensitivity
Ordering Info
| Size | Catalog |
|---|---|
| 100 U | 600420 |
| 500 U | 600422 |
| 1000 U | 600424 |
| Master Mix (100 rxn) | 600650 |
Herculase II Fusion DNA Polymerase
Product Details
- Superior yield for routine PCR applications & difficult/GC-rich targets
- Fast cycling time with 15 sec/kb extension rate
- Accurately amplifies up to 23 kb genomic DNA targets
-
Preserves Pfu fidelity,
1 error/770,000 bp - High sensitivity for amplification of low abundance targets
Ordering Info
| Size | Catalog |
|---|---|
| 40 rxn | 600675 |
| 200 rxn | 600677 |
| 400 rxn | 600679 |
Other
What do you value the most in DNA Polymerase?
Accuracy
What is your preferred format?
Hot Start Master Mix
Recommendation:
PfuUltra II HotStart Master Mix
Product Details
- Highest fidelity for the most reliable, error-free PCR
- Fast cycling time with 15 sec/kb extension rate
- Accurately amplifies up to 19 kb genomic DNA targets
- Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
| Size | Catalog |
|---|---|
| 100 rxn | 600850 |
| 400 rxn | 600852 |
Hot Start Enzyme
Recommendation:
PfuUltra II Fusion HotStart DNA Polymerase
Product Details
- Highest fidelity for the most reliable, error-free PCR
- Fast cycling time with 15 sec/kb extension rate
- Accurately amplifies up to 19 kb genomic DNA targets
- Ideal for applications such as cloning, protein crystallization studies & sequencing
Ordering Info
| Size | Catalog |
|---|---|
| 40 rxn | 600670 |
| 200 rxn | 600672 |
| 400 rxn | 600674 |
Robustness/Yield
Recommendation:
Herculase II Fusion DNA Polymerase
Product Details
- Superior yield for routine PCR applications & difficult/GC-rich targets
- Fast cycling time with 15 sec/kb extension rate
- Accurately amplifies up to 23 kb genomic DNA targets
- Preserves Pfu fidelity, 1 error/770,000 bp
- High sensitivity for amplification of low abundance targets
Ordering Info
| Size | Catalog |
|---|---|
| 40 rxn | 600675 |
| 200 rxn | 600677 |
| 400 rxn | 600679 |
Fidelity
Recommendation:
PfuUltra II Fusion Hot Start DNA Polymerase
Product Details
- Highest fidelity with great PCR success and reliability
- 70 to 80 % quicker time to result
- High specificity hotstart formulation
- Amplify up to 19 kb genomic DNA
- Ideal for PCR cloning, RT-PCR, and site-directed mutagenesis
Ordering Info
| Size | Catalog |
|---|---|
| 40 rxn | 600670 |
| 200 rxn | 600672 |
| 400 rxn | 600674 |
Non-Proofreading
What is your preferred format?
Hot Start Master Mix
Recommendation:
Paq5000 2X PCR Master Mix
Product Details
- Robust, cost effective alternative to Taq
- Faster cycling times with 30 sec/kb extension rate
- Amplify up to 6 kb genomic DNA targets
Ordering Info
| Size | Catalog |
|---|---|
| 100 rxn | 600870 |
| 400 rxn | 600872 |
Hot Start Enzyme
Recommendation:
SureStart Taq DNA Polymerase
Product Details
- Hot start for greater PCR specificity and yields
- Virtually eliminates background artifacts and smearing in long PCR
- Reliable room-temperature setup
- Ultrapure cloned Taq DNA polymerase
Ordering Info
| Size | Catalog |
|---|---|
| 100 U | 600280 |
| 500 U | 600282 |
| 1000 U | 600284 |
Stand Alone Enzyme
Recommendation:
Taq2000 DNA Polymerase
Product Details
- Ultrapure cloned Taq DNA polymerase
- Virtually eliminates background artifacts and smearing in long PCR
- Most thermostable Taq DNA polymerase
Ordering Info
| Size | Catalog |
|---|---|
| 100 U | 600195 |
| 500 U | 600196 |
| 1000 U | 600197 |
| 5000 U | 600198 |
Welcome to the Polymerase Decision Tree!
This decision tree is your personal guide through Agilent's PCR enzyme portfolio. In just a few clicks, find out which polymerase best fits your application.
GET STARTED!