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Millipore Detect Cnpase Using This Milli-Mark Anti-Cnpase-Fitc Antibody, Clone 11-5b Validated For Use In Fc. - Mill (Additional S&H or Hazmat Fees May Apply)
CNPase (2, 3-cyclic nucleotide 3-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Product Information
Format
FITC
Control
U251 Cells
Presentation
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality Level
MQ100
Applications
Application
Detect CNPase using this Milli-Mark Anti-CNPase-FITC Antibody, clone 11-5B validated for use in FC.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Purified human brain CNPase protein.
Clone
11-5B
Host
Mouse
Specificity
Antibody recognizes Human CNPase protein.
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_149124
Gene Symbol
CNP
CNPase
CNP1
EC 3.1.4.37
Purification Method
Protein A Purfied
UniProt Number
P09543
UniProt Summary
SIZE: 421 amino acids; 47579 Da
SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.
Molecular Weight
48kDa Calculated
Product Usage Statements
Quality Assurance
Evaluated by flow cytometry using U251 Cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
