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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB3852 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cortactin Antibody, phospho-specific (Tyr421) |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified immunoglobulin. Liquid in 1mg/mL in PBS with 0.05% Bronidox. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cortactin Antibody, phospho-specific (Tyr421) detects level of Cortactin & has been published & validated for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 1-2 μg/mL Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Rabbit anti-Cortactin (Y421) polyclonal antibody was raised against a peptide corresponding to residues surrounding phosphorylated Y421of human cortactin. |
| Epitope | phospho-specific (Tyr421) |
| Host | Rabbit |
| Specificity | Recognizes Cortactin phosphorylated on tyrosine 421. Cortactin is an 80-85 kDa actin-binding protein, which consists of a variety of protein domains. The nature and function of these domains, coupled with the presence of multiple consensus sites for phosphorylation by tyrosine and serine/threonine kinases, suggests that cortactin integrates activation of specific signaling pathways with cytoskeletal reorganization. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. |
| Gene Symbol |
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| Modifications |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q14247 # May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. SIZE: 550 amino acids; 61636 Da SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity). SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months after receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |