Sigma-Aldrich Grams Decolorizing Soluti

Quality Level

400

Form

liquid

Autoignition Temp.

>425 °C

Ivd

forin vitrodiagnostic use

Transition Temp

flash point -10 °C

Density

0.79 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

15-25°C

Related Categories

Bacteriology Stains

Hematology Stains

General Description

Gram′s decolorizing solution - for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodine solution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution (Gram′s safranine solution (Product number 1.09217) and appear pink to red.
A bottle with 500 ml Gram′s decolorizing solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU).

Analysis Note

Suitability for microscopy: passes test
Staphylococcus aureus: dark blue-violet
E.coli: pink to red

Pictograms

GHS02,GHS07

Signalword

Danger

Hcodes

H225 - H319 - H336

Pcodes

P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target_Organs

Central nervous system

Storage Class

3 - Flammable liquids

Wgk_Germany

WGK 1

Flash_Point_F

14.0 °F

Flash_Point_C

-10 °C