Sigma-Aldrich Lugols Solution Stabilize

Quality Level

400

Form

liquid

IVD

forin vitrodiagnostic use

pH

2.0 (20-22 °C in H₂O)

Density

1.02 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

15-25°C

Related Categories

Bacteriology Stains

Hematology Stains

General Description

The Lugol′s solution stabilized with PVP, is a ready-to-use solution used in bacteriology, for example, for the staining of Gram bacteria. It contains a complex of Iodine-Polyvinylpyrrolidon (PVP) and no free iodine, which stabilizes the solution and reduces the evaporation of iodine. During Gram-staining, the iodine from the Lugol′s solution and crystal violet (Cat. no. 109218) form a stable complex that will not be released by decolorisation. Thus, Gram-positive bacteria will appear blue-violet when analyzed microscopically, wheras Gram-negative bacteria can be counterstained with a safranine solution (Product number 1.09217) and will appear pink to red.
1 L of the Lugol′s solution stabilized with PVP is capable of staining up to 500 slides. This product is registered as IVD and CE marked.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
gram-positve microorganisms: blue-violet
gram-negative microorganisms: pink to red

Pictograms

GHS05

Signalword

Danger

Hcodes

H318 - H412

Pcodes

P273 - P280 - P305 + P351 + P338 - P501

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class

12 - Non Combustible Liquids

Wgk_Germany

WGK 2

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable