Sigma-Aldrich Wrights Eosin Methylene B

Quality Level

400

Form

liquid

Autoignition Temp.

420 °C (Methanol)

Expl. Lim.

5.5-44 % (v/v) Methanol)

Ivd

forin vitrodiagnostic use

Ph

7-8 (20 °C in H₂O)

Transition Temp

flash point 9.7 °C (Methanol)

Density

0.80 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

15-25°C

Related Categories

Hematology Stains

General Description

The Wright′s eosin methylene blue solution for microscopy, is a ready-to-use solution used in the staining method according to Wright, is one of the standard techniques in hematological diagnostic procedures. Like Leishman′s eosin methylene blue solution and May-Grünwald′s eosin methylene blue solution, it is used for staining of blood and bone marrow smears and clinical cytological specimens.
The Wright′s eosin methylene blue solution is an IVD / CE product and 500 ml are sufficient for staining up to 1000 slides in Hellendahl cells. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy (Blood smear): passes test
Erythrocytes: pink to brownish
Nuclei: blue-violet
Eosinophilic granules: red to red-brown
Neutrophilic granules: light violet
Lymphocyte cytoplasm: blue

Pictograms

GHS02,GHS06,GHS08

Signalword

Danger

Hcodes

H225 - H301 + H311 + H331 - H370

Pcodes

P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Storage Class

3 - Flammable liquids

Wgk_Germany

WGK 2

Flash_Point_F

49.5 °F - Methanol

Flash_Point_C

9.7 °C - Methanol