Sigma-Aldrich Brilliant Cresyl Blue Solu

Quality Level

400

Form

liquid

Ivd

forin vitrodiagnostic use

Ph

3.7 (20 °C in H₂O)

Density

1.01 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

15-25°C

Related Categories

Hematology Stains

General Description

The Brilliant cresyl blue solution - for the staining of reticulocytes - for microscopy, is a ready-to-use solution used for human-medical cell diagnosis and serves the hematological investigation of sample material of human origin.
The dye stains the nucleic acids in the reticulocytes (Substantia granulofilamentosa), thus enabling reticulocytes to be morphologically distinguished from erythrocytes. The Substantia granulofilamentosa (ribonucleoproteins) can be rendered visible with fresh, non-fixed, young erythrocytes in a supervital staining procedure. Characterized by an intense black-blue hue, it is possible both to determine the number of the reticulocytes (visual microscopic counting) and also to visualize their morphological aspects. Four stages of substantia granulofilamentosa maturation can be distinguished depending on the stage of reticulocyte development: coiled skein (I), incomplete network (II), complete network (III) and granular form (IV). In peripheral blood the development stages III and IV are found most commonly.
The 100 ml bottle provides ca. 1000 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Application test (Reticulocyte staining): passes test

Storage Class

12 - Non Combustible Liquids

Wgk_Germany

WGK 2

Flash_Point_F

Not applicable

Flash_Point_C

Not applicable