Sigma-Aldrich Hematoxylin Solution Modif

Quality Level

400

Form

liquid

IVD

forin vitrodiagnostic use

pH

2.5 (20 °C in H₂O)

Density

1.07 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

15-25°C

Related Categories

Cytology Stains

Hematology Stains

General Description

Hematoxylin solution modified according to Gill III - for microscopy, is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin. It is a ready-to-use staining solution that when used together with other in vitro diagnostic products from our portfolio makes target structures evaluable for diagnostic purposes in histological and clinico-cytological specimen materials, for example histological sections of e. g. the kidney, muscle tissue, heart, or lung. In hematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready-to-use staining solutions, such as Mayer′s hemalum solution or Hematoxylin solution modified according to Gill III. These solutions do not need to be filtrated prior use and the dye is oxidized in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e. for proteins, collagen, keratin, intercellular substances. can be performed with alcoholic or aqueous Eosin Y solution 0.5 %.
A 500 mL bottle is sufficient for 1500 - 2500 stainings. It is registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Nuclei: dark blue to dark violet
Cytoplasm: pink to red
intercellular substances: pink to red
Erythrocytes: yellow to orange

Pictograms

GHS05,GHS08

Signalword

Danger

Hcodes

H318 - H373

Pcodes

P260 - P280 - P305 + P351 + P338 - P314 - P501

Hazard Classifications

Eye Dam. 1 - STOT RE 2 Oral

Target_Organs

Kidney

Storage Class

10 - Combustible liquids

Wgk_Germany

WGK 1