Sigma-Aldrich Weigerts Iron Hematoxylin

Quality Level

400

IVD

forin vitrodiagnostic use

Application(S)

clinical testing
diagnostic assay manufacturing
hematology

Storage Temp.

15-25°C

Related Categories

Cytology Stains

Hematology Stains

General Description

Weigert′s iron hematoxylin kit - for nuclear staining in histology is a ready to use staining kit used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin, for example histological sections of e. g. the liver, the kidney, the intestine, the placenta and similar. Aluminium hematoxylins (e. g. nuclear fast red aluminium sulfate) are washed out of the tissue sections by acidic staining solutions. Iron hematoxylin solutions (e. g. Weigert′s iron hematoxylin), on the other hand, are acid-resistant and are preferentially used for staining nuclei in histological tissue sections in which counterstaining is done with acidic staining solutions. Examples here include the visualization of elastic fibers according to the van Gieson method (e. g. Elastica van Gieson (Product number 1.15974)) and the various trichromo connective-tissue staining methods (e. g. according to Masson-Goldner (Product number 1.00485). Commonly used hemalum solutions such as Mayer′s Hemalum, Delafield, Gill and Harris tend to procedure also weak nucleic staining only when used with acidic counter-stains.The kit contains two solutions; an alcoholic hematoxylin solution and a hydrochloric acid iron(III)nitrate solution, which are ready-to-use and should be applied at 3-5 μm paraffin sections.
The package is sufficient for 400 - 500 applications. Weigert′s Iron hematoxylin kit is an IVD & CE product and can be used in the clinical laboratory for diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Pictograms

GHS02,GHS05

Signalword

Danger

Hcodes

H225 - H290 - H319

Pcodes

P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1

Storage Class

3 - Flammable liquids

Wgk_Germany

WGK 1