Sigma-Aldrich Eosin Y Solution 1% Alcoho

Quality Level

400

Form

liquid

Ivd

forin vitrodiagnostic use

Transition Temp

flash point 19 °C

Density

0.892 g/cm³at 20 °C

Application(S)

clinical testing
diagnostic assay manufacturing
hematology
histology

Storage Temp.

15-25°C

Related Categories

Hematology Stains

General Description

Eosin Y solution 1%, alcoholic - for microscopy is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin in histological and clinico-cytological specimen materials, for example histological sections of e. g. the kidney, muscle tissue, heart, or lung. The hematoxylin and eosin (H&E) staining method is the method most frequently used for the staining of histology material. The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (hematoxylin) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasma and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color.
One 500ml bottle of the stain provides 1250 stainings. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

Analysis Note

Suitability for microscopy (Tissue section): passes test
Nuclei: dark blue to dark violet
Cytoplasm: pink to red
intercellular substances: pink to red
Erythrocytes: yellow to orange

Pictograms

GHS02,GHS07

Signalword

Danger

Hcodes

H225 - H317 - H319

Pcodes

P210 - P233 - P240 - P280 - P303 + P361 + P353 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

Storage Class

3 - Flammable liquids

Wgk_Germany

WGK 2

Flash_Point_F

66.2 °F

Flash_Point_C

19 °C