Takara Tet-On 3G Bidirectional Inducible Expression System \Each

Takara The Tet-On 3G Bidirectional Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of two genes of interest from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI Vector Set, consisting of a bidirectional vector into which both genes of interest can be cloned and simultaneously expressed, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS. - TAKBGN (Additional S&H or Hazmat Fees May Apply)