Sigma-Aldrich Colcemid

Description

N-methyl-N-deacetyl-colchicine

Quality Level

100

Sterility

sterile; 0.2 μm filtered

Form

solution

Packaging

pkg of 20 mL (10 μg/mL)

Manufacturer/Tradename

Roche

Technique(S)

blocking: suitable

Impurities

microbial, tested

Solubility

water: miscible

Storage Temp.

2-8°C

Related Categories

Roche® Life Science Products

General Description

Colcemid is also known as demecolcine. Its generic name isN-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase.

Application

Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis.

Biochem/Physiol Actions

Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized g₁and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

Physical Form

Solution (10 μg/mL), filtered through 0.2 μm pore size membrane.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

Wgk_Germany

nwg

Flash_Point_F

does not flash

Flash_Point_C

does not flash