GenScript Protein G Magbeads Mx; 4mL (1 mL Settled Beads)

Designed Use
GenScript Protein G MagBeads MX are ideal for small-scaleantibody purification.

Principle
The sample containing antibody is added to theProtein G MagBeads MX. The antibody will bind to beads during a shortincubation. Then the bead-bound antibody may be eluted off the beads by using amagnetic separation rack. Magnetic separation eliminates the need for multipletubes, minimizes the loss of sample and removes tedious steps of conventionalcentrifugation method.

Material Supplied
GenScript Protein G MagBeads MX are superparamagneticbeads of average 45±5 μm in diameter, covalently coated with recombinant Protein G. The beadsare supplied as 25% slurry in 20% ethanol. The Protein G MagBeads MX have abinding capacity of more than 25 mg Human IgG per 1 ml settled beads (e.g. 4 ml25% slurry).
Protein G is a genetically engineered protein(MW≈22 kDa) that combines the IgG binding sites of both Protein A and ProteinG. 6×His-tag was attached to its N-terminal to facilitate the purification.Protein G has greater affinity than protein A for most mammalian IgGs,especially for certain subclasses including human IgG3, mouse IgG1 and ratIgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Storage and Stability
This product is stable until the expiration datestated on the label, when stored unopened at 2–8°C. Do not freeze the product. Keep the MagBeadsin liquid suspension during storage and all handling steps. Drying will causeloss of binding capacity and result in reduced performance. Resuspend the beadswell before use. Be careful to avoid bacterial/fungal contamination.