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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H | ICC, IHC | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB15708A4 |
| Description | Anti-Beta III Tubulin Antibody, Alexa Fluor® 488 Conjugate |
| Alternate Names |
|
| Background Information | Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified rabbit polyclonal conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Beta III Tubulin Antibody, Alexa Fluor 488 Conjugate is an antibody against Beta III Tubulin for use in IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: Representive lot has been shown to work in adult mouse brain. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic linear peptide corresponding to human Beta III Tubulin. |
| Host | Rabbit |
| Specificity | This antibody recognizes Beta III Tubulin. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Rat and Mouse. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 450 amino acids; 50433 Da SUBUNIT: Dimer of alpha and beta chains. DOMAIN: The highly acidic C-terminal region may bind cations such as calcium. SIMILARITY: Belongs to the tubulin family. |
| Molecular Weight | 50 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in rat E18 cortex cells. Immunocytochemistry Analysis: A 1:400 dilution of this antibody detected Beta III Tubulin in rat E18 cortex cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |