Cell Signaling Cleaved Caspase-3 (Asp175) (D3e9) Rabbit mAb (PE Conjugate)

Name: Cell Signaling Cleaved Caspase-3 (Asp175) (D3e9) Rabbit mAb (PE Conjugate)
SKU: CSIG-12768S
Price: 497.93 USD

List Price $497.93 Your Price $497.93

Cell Signaling Cleaved Caspase-3 (Asp175) (D3e9) Rabbit mAb (PE Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-12768S

MFG.PART: 12768S

UNSPSC: 12352202

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (PE Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. This antibody may also detect non-specific caspase substrates. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Nuclear background may be observed in rat and monkey samples.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.
Background	Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).