Cell Signaling Bcl-2 (D55g8) Rabbit mAb (Human Specific)

Name: Cell Signaling Bcl-2 (D55g8) Rabbit mAb (Human Specific)
SKU: CSIG-4223T
Price: 153.52 USD

List Price $153.52 Your Price $153.52

Cell Signaling Bcl-2 (D55g8) Rabbit mAb (Human Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4223T

MFG.PART: 4223T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	26
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Bcl-2 (D55G8) Rabbit mAb detects endogenous levels of total Bcl-2 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His58 of human Bcl-2.
Background	Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

Specifications