Cell Signaling Cleaved Caspase-8 (Asp387) (D5b2) Xp Rabbit mAb (Mouse Specific)

Name: Cell Signaling Cleaved Caspase-8 (Asp387) (D5b2) Xp Rabbit mAb (Mouse Specific)
SKU: CSIG-8592T
Price: 180.79 USD

List Price $180.79 Your Price $180.79

Cell Signaling Cleaved Caspase-8 (Asp387) (D5b2) Xp Rabbit mAb (Mouse Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-8592T

MFG.PART: 8592T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	M
Sensitivity	Endogenous
Molecular Weight (kDa)	18, 43
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600, Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #97756.
Specificity/Sensitivity	Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, this antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43).
Species Reactivity	Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.
Background	Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.