Cell Signaling Cleaved Caspase-8 (Asp374) (18c8) Rabbit mAb

Name: Cell Signaling Cleaved Caspase-8 (Asp374) (18c8) Rabbit mAb
SKU: CSIG-9496T
Price: 164.63 USD

List Price $164.63 Your Price $164.63

Cell Signaling Cleaved Caspase-8 (Asp374) (18c8) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9496T

MFG.PART: 9496T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	18, 41, 43
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved Caspase-8 (Asp374) (18C8) Rabbit mAb detects human Caspase-8 when cleaved at Asp374. This antibody will detect cleavage products containing the pro-domain with the p18 subunit as well as the p18 subunit alone. A band of unknown origin is detected at 25 kDa in some cell lines.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp374 of human Caspase-8.
Background	Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

Specifications