Cell Signaling Caspase-9 Antibody (Human Specific)

Name: Cell Signaling Caspase-9 Antibody (Human Specific)
SKU: CSIG-9502T
Price: 132.31 USD

List Price $132.31 Your Price $132.31

Cell Signaling Caspase-9 Antibody (Human Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-9502T

MFG.PART: 9502T

UNSPSC: 12352202

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	35, 37, 47
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Caspase-9 Antibody detects endogenous levels of full length caspase-9 (47 kDa) and large fragments of caspase-9. The antibody does not recognize other caspases.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding aspartic acid 315 of human caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Specifications