Revvity PhenoVue 577 Lysosomal stain

Revvity Lysosomes are abundant organelles which contain more than sixty acidic enzymes. Lysosomes play essential role in macromolecules and organelles break down through the autophagy-lysosomal system. Defective regulation of lysosomal function has been reported in several diseases such as lysosomal storage disorders and neurodegenerative diseases like Alzheimer’s and Parkinson’s. PhenoVue 577 lysosomal stain is partially protonated at neutral pH, and highly protonated at acidic pH. In living cells, PhenoVue 577 lysosomal stain accumulates in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining. PhenoVue 577 lysosomal stain can be used to lysosomes in immunofluorescence, as well as high-content analysis and screening applications. Features Numbers of Vials Per Unit 20 Quantity or Volume Per Unit 19.9µg (50nmoles) Form Solution 1mM DMSO Storage -16°C or below Recommended Working Concentration 50 ng/mL Maximum Excitation Wavelength 577 nm Maximum Emission Wavelength 590 nm Common Filter Set RFP Live Cell Staining Yes Fixed Cell Staining No Equivalent Number of Microplates 650 - 2050 x 96-well microplates 550 - 2050 x 384-well microplates 1050 - 3250 x 1536-well microplates - PEMSD